Dual Affiliation with Division of Biology and Biological Engineering
Assistant: Cheryl Nakashima
Research in the Arnold group focuses on evolutionary protein design methods (1,2) and using the results of laboratory evolution experiments to elucidate principles of biological design.
We generate novel and useful enzymes and organisms for applications in medicine, neurobiology, chemical synthesis and alternative energy. We also construct entire synthetic families of enzymes and other proteins in order to study structure-function relationships free from constraints of natural selection.
This research requires contributions from many disciplines, including chemistry, bioengineering, biochemistry, molecular biology, microbiology, chemical engineering, chemistry and applied physics.
Publications
- Alfonzo, Edwin;Hanley, Deirdre et al. (2024) Biocatalytic Synthesis of α-Amino Esters via Nitrene C–H InsertionJournal of the American Chemical Society
- Hanley, Deirdre;Li, Zi-Qi et al. (2024) Stereospecific Enzymatic Conversion of Boronic Acids to AminesJournal of the American Chemical Society
- Almhjell, Patrick J.;Johnston, Kadina E. et al. (2024) The β-subunit of tryptophan synthase is a latent tyrosine synthaseNature Chemical Biology
- Mao, Runze;Gao, Shilong et al. (2024) Biocatalytic, enantioenriched primary amination of tertiary C–H bondsNature Catalysis
- Yang, Jason;Li, Francesca-Zhoufan et al. (2024) Opportunities and Challenges for Machine Learning-Assisted Enzyme EngineeringACS Central Science
- Rogge, Torben;Zhou, Qingyang et al. (2024) Iron Heme Enzyme-Catalyzed Cyclopropanations with Diazirines as Carbene Precursors: Computational Explorations of Diazirine Activation and Cyclopropanation MechanismJournal of the American Chemical Society
- Sarai, Nicholas S.;Fulton, Tyler J. et al. (2024) Directed evolution of enzymatic silicon-carbon bond cleavage in siloxanesScience
- Yang, Jason;Ducharme, Julie et al. (2024) Correction to "DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein Engineering"ACS Synthetic Biology
- Wackelin, Daniel J.;Mao, Runze et al. (2024) Enzymatic Assembly of Diverse Lactone Structures: An Intramolecular C–H Functionalization StrategyJournal of the American Chemical Society
- Qin, Zi-Yang;Gao, Shilong et al. (2023) Biocatalytic Construction of Chiral Pyrrolidines and Indolines via Intramolecular C(sp³)–H AminationACS Central Science